產(chǎn)品貨號(hào)：K-PANOPA
品牌：Megazyme
產(chǎn)品品名：一級(jí)氨基氮[NOPA]檢測(cè)試劑盒
英文品名：Primary Amino Nitrogen (PANOPA) Assay Kit
規(guī)格型號(hào)：100 assays (manual) / 1000 assays (microplate) / 1100 assays (auto-analyser)
For the assay of primary amino nitrogen in grape juice/must and wine.
INTRODUCTION:
The addition of nutrient supplements to grape juice prior to/during
fermentation can now be managed accurately in terms of Yeast
Available Nitrogen (YAN). YAN is especially important to determine
accurately, as too little available nitrogen can result in sluggish or
“stuck” fermentation, and the generation of hydrogen sulphide
(H2S) gas, while too much nitrogen, such as in the form of added
diammonium phosphate (DAP), can lead to the formation of the
carcinogenic compound ethyl carbamate, especially where starting
levels of L-arginine in the juice are high.
The PAN assay procedure (K-PANOPA) described in this booklet
is a more accurate alternative to formol titration that also measures
proline, an abundant amino acid found in grape juice that does not
contribute to YAN in anaerobic fermentations.
KITS:
Kits suitable for performing 100 assays in manual format (or 1100
assays in auto-analyser format or 1000 assays in microplate format) are
available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Tablets (100) containing N-acetyl-L-cysteine (NAC).
Stable for > 2 years at 4°C or -20°C.
Bottle 2: Ortho-phthaldialdehyde (OPA) in 12 mL of ethanol
(96% v/v).
Stable for > 2 years at 4°C.
Bottle 3: Isoleucine standard solution (5 mL, 140 mg of
nitrogen/L).
Stable for > 2 years at 4°C.
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