產(chǎn)品貨號：K-ARGA 

品牌：Megazyme

產(chǎn)品品名：L-阿拉伯糖D-半乳糖檢測試劑盒

英文品名：L-Arabinose / D-Galactose Assay Kit

規(guī)格型號：115 assays (manual) / 1150 assays (microplate) / 1150 assays (auto-analyser) 

A simple, reliable and accurate UV method for the measurement ofL-arabinose and/or D-galactose in various materials including foods,feeds, beverage and plant products. 

INTRODUCTION:

Enzymic kits for the determination of L-arabinose and D-galactose
are very slow. This is due to the low rate of natural chemical
“mutarotation” between the α- and β-anomeric forms of these
monosaccharides. Only the β-form is recognised by β-galactose
dehydrogenase. In incubations containing NAD+, L-arabinose
or D-galactose and β-galactose dehydrogenase, there is a very
rapid initial increase in absorbance due to the consumption of β
-L-arabinose or β-D-galactose, and this is followed by a very slow
approach to the end-point. This very slow approach results from
the very low rate of chemical “mutarotation” of the α-anomeric
conformation of these monosaccharides to the respective β-anomer.
Using technology developed by Megazyme (patent pending), a
galactose mutarotase has now been incorporated into the assay
format to rapidly catalyse this rate-limiting mutarotation step.
The result is very rapid analysis times of approximately 6 min for
D-galactose and approximately 12 min for L-arabinose (Figure 1).

KITS:

Kits suitable for performing 115 assays in manual format (or 1150 assays
in microplate or auto-analyser formats) are available from Megazyme.
The kits contain the full assay method plus:
Bottle 1: Buffer (25 mL, pH 8.6) plus sodium azide (0.02% w/v) as
a preservative. Stable for > 2 years at 4°C.
Bottle 2: NAD+. Lyophilised powder.
Stable for > 5 years at -20°C.
Bottle 3: β-Galactose dehydrogenase plus galactose mutarotase
suspension, 2.4 mL. Stable for > 2 years at 4°C.
Bottle 4: L-Arabinose standard solution (5 mL, 0.4 mg/mL in
0.02% w/v sodium azide).
Stable for > 2 years at 4°C.
Bottle 5: D-Galactose standard solution (5 mL, 0.4 mg/mL in
0.02% w/v sodium azide).
Stable for > 2 years at 4°C.


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