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Certificate of Analysis and Data Sheet Mouse Anti maltose-binding protein (MBP) |
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Antigen: |
The malE gene is inserted in a pMAL vector and then expressed in E. coli JM109. | |
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Specificity: |
Mouse monoclonal antibody against the amino acid stretch of KDPRIAA (the amino acid residue 339 to 345) of the periplasmic maltose-binding protein (MBP) [Escherichia coli] (Accession #: AAB59056). | |
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Antibody Formulation: |
Mouse Anti maltose-binding protein is lyophylized at a concentration of 1 µg/µl from ammonium sulfate-precipitated ascites fluid ,PBS with 1% BSA. | |
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Storage Procedure: |
Lyophilized: store at 4 C. After reconstitution, if not used within a month, aliquot and store at 20 C. If more dilute solution required, it is suggested that they be made in a buffered solution containing carrier protein such as PBS with 0.1% BSA. | |
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Reconstitution: |
Reconstitute with 1 mg/ml of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. | |
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Stability / Shelf life: |
Two years lyophilized, two months in solution at 4 C. | |
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Applications : |
1. Immunofluorescence: Typical working dilution on frozen sections 1:200. 60 minutes primary antibody incubates at 25ºC. 2. Western blotting: Recommended: working dilution on Western blotting 1:500. 60 minutes primary antibody incubates at 25ºC. | |
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Usage: |
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |