HepaOneTM
Recombinant Human Heparanase-1 (rhHPA1)
Stabilized, ed, Lyophilized

Catalog Number: INS-26-0-0001-93 (1 μg)
INS-26-0-0001-90 (4 μg)
INS-26-0-0001-91 (8 μg)
BACKGROUND
Heparanase is an endo-β-D-glucuronidase, which degrades
heparan sulfate side chains of heparan sulfate proteoglycans
(HSPGs) in the extracellular matrix. Heparanase plays an
important role in ECM degradation, facilitating the migration and
extravasation of tumor cells and inflammatory leukocytes (1,2,3).
Upon degradation, Heparanase releases growth factors and
cytokines that stimulate cell proliferation and chemotaxis (4,5).
Heparanase is a glycosylated heterodimer comprised of a 50-kDa
subunit, harboring the active site, and an 8 kDa subunit. It is
produced as a latent 65-kDa precursor and proteolytically
processed to its active form (1,6).
Heparanase is highly expressed in myeloid leukocytes (i.e.
neutrophils) in platelets and in human placenta. Human heparanase
was found to be upregulated in various types of primary tumors,
correlating in some cases with increased tumor invasiveness and
vascularity and with poor prospective survival (7,8).
SOURCE
HepaOneTM is produced in CHO cells. The rhHPA1 protein was
purified by several orthogonal chromatography steps.
IDENTITY
HepaOneTM is identified by Western blot analysis with polyclonal
rabbit anti-HPA1 antibodies (Cat. # INS-26-2-0000-11) as 2
subunits of 8-kDa and 50-kDa (Fig. 1, A).
PURITY
>95% on SDS-PAGE (Fig. 1, B)
>95% on RP-HPLC (Fig. 1, C)
POTENCY
The enzymatic activity of HepaOneTM was determined by activity
assay in which immobilized heparin, released due to heparanase
activity, is quantified colorimetrically and was found to comply
with rhHPA1 standard. Recommended reaction buffer: 20 mM
citrate phosphate; pH 5.4
PRODUCT
Each vial of HepaOneTM contains 10 μg d-Mannitol; 10 μg
Human Serum Albumin; and 100 μg Glycine per 1 μg of rhHPA1
in a buffer containing 0.612 μg Acetic Acid and 4.04 μg Sodium
Acetate (anhydrous) as lyophilized powder.
RECONSTITUTION
It is recommended to reconstitute the lyophilized rhHPA1 in sterile
18MΩ-cm water at a concentration of not less than 300 μg/ml,
which can then be further diluted to other aqueous solutions.
STORAGE
Store at 2-8°C. When stored appropriately, HepaOneTM is stable
for 6 months from the date of shipment. Upon reconstitution,
HepaOneTM may be kept at 4°C for one month. Avoid freezing
and thawing cycles.

http://www.insight.co.il/files/rhhpa1sl%20_2006.pdf