Monoclonal Anti-Human Heparanase 1 (HPA1) Antibody
Clone HP3/17

Catalog Number: INS-26-1-0000-10 (50 μg)
INS-26-1-0000-11 (100 μg)
INS-26-1-0000-12 (150 μg)
BACKGROUND
Heparanase is an endo-β-D-glucuronidase, which degrades heparan
sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the
extracellular matrix. Heparanase plays an important role in ECM
degradation, facilitating the migration and extravasation of tumor
cells and inflammatory leukocytes (1,2,3). Upon degradation,
heparanase releases growth factors and cytokines that stimulate cell
proliferation and chemotaxis (4,5).
Heparanase is a heterodimer comprised of a 50 kDa subunit
harboring the active site and a 8 kDa subunit. It is produced as a
latent 65 kDa precursor and proteolytically processed to its active
form (1,6).
Heparanase is highly expressed in myeloid leukocytes (i.e.
neutrophils) in platelets and in human placenta. Human heparanase
was found to be upregulated in various types of primary tumors,
correlating in some cases with increased tumor invasiveness and
vascularity and with poor prospective survival (7,8).
SOURCE
Mab HP3/17 is a Protein G affinity purified monoclonal antibody
raised against a polypeptide from the 50 kDa subunit of Heparanase.
Ig CLASS
Mouse IgG2Bκ
PRODUCT
Each vial contains 50, 100 or 150 μg in 12.5, 25 or 37.5 μl
respectively, of 0.22 micron filtered solution of 20 mM Sodium
Phosphate; 150 mM NaCl; pH 7.2, containing 0.01% Thimerosal.
APPLICATIONS
Western blot
Immunohistochemistry
SPECIFICITY
HP3/17 reacts with the 50 kDa subunit and with the 65 kDa precursor
of human or mouse Heparanase by Western blotting and
immunohistochemistry.
Recommended dilution range for Western blot analysis: 1:4000.
Recommended dilution range for immunohistochemistry: 1:40.
PURITY
>98% on SDS-PAGE when loaded 50 μg/lane.
STORAGE
Store at 4°C. Stable for six months from the date of shipment. For
extended storage, freeze in working aliquots at -20°C. Avoid
repeated freeze-thaw cycles.
RESEARCH USE
For in vitro research use only. Not for use in diagnostic
procedures.

http://www.insight.co.il/files/mab%20hp3-17_2006.pdf