Polyclonal Rabbit Anti-Human Heparanase 1 (HPA1) Antibody

Catalog Number: INS-26-2-0000-11 (0.24 mg)
INS-26-2-0000-12 (0.48 mg)
BACKGROUND
Heparanase is an endo β-D-glucuronidase, which degrades heparan
sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the
extracellular matrix. Heparanase plays an important role in ECM
degradation, facilitating the migration and extravasation of tumor
cells and inflammatory leukocytes (1,2,3). Upon degradation,
heparanase releases growth factors and cytokines that stimulate cell
proliferation and chemotaxis (4,5). Heparanase is a heterodimer
comprised of a 50 kDa subunit harboring the active site and a 8
kDa subunit. It is produced as a latent 65 kDa precursor and
proteolytically processed to its active form (1,6). Heparanase is
highly expressed in myeloid leukocytes (i.e. neutrophils) in
platelets and in human placenta. Human heparanase was found to
be upregulated in various types of primary tumors, correlating in
some cases with increased tumor invasiveness and vascularity and
with poor prospective survival (7,8).
SOURCE
Polyclonal rabbit anti-human HPA1 is a Protein G affinity purified
polyclonal antibody raised against the 50 kDa-8 kDa Heparanase
heterodimer.
PRODUCT
Each vial contains 0.24 mg or 0.48 mg of antibody in 100 or 200
μL respectively of 20 mM sodium phosphate; 150 mM NaCl; pH
7.2, containing 0. 01% Thimerosal.
APPLICATIONS
Western blot
Immunohistochemistry
SPECIFICITY
Western blot analysis: The antibody reacts with the 65 kDa
precursor as well as the 50 kDa and 8 kDa subunits of human or
mouse Heparanase.
Immunohistochemistry: The antibody interacts with Heparanase in
paraffin sections and blood smears.
Recommended dilution range for Western blot analysis: 1:1000.
Recommended dilution range for immunohistochemistry: 1:100.
STORAGE
Store at 40C. For extended storage, freeze in working aliquots at
-200C.
Avoid repeated freeze-thaw cycles.
RESEARCH USE
For in vitro research use only. Not for use in diagnostic procedures.
PATENTS
Polyclonal and monoclonal Anti-heparanase antibodies and their
uses are protected by US. Patents No. 6,177,545; 6,531,129,
additional US patent applications and patents and patent
applications worldwide.

http://www.insight.co.il/files/poly%20anti-%20hpa1_2006.pdf