Total Human Coagulation Factor XI ELISA
For Research Use Only
INTRODUCTION
This assay is intended for the quantitative determination of total Factor XI antigen in human plasma.
Factor XI is a disulfide linked two-chain glycoprotein zymogen and is the precursor of the coagulation
enzyme Factor XIa1. Factor XI consists of two identical monomers and circulates in plasma in complex
with kininogen2. Factor XI is activated by Factor XIIa and converts Factor IX to Factor IXa during the
intrinsic pathway of the coagulation cascade3.
PRINCIPLES OF PROCEDURE
Human Factor XI will bind to the affinity purified capture antibody coated on the microtiter plate. Factor
XI and XIa will react with the antibody on the plate. After appropriate washing steps, peroxidase labeled
polyclonal anti-human Factor XI primary antibody binds to the captured protein. Excess antibody is
washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is
prepared along with the samples to be measured using dilutions of human Factor XI. Color development
is proportional to the concentration of Factor XI in the samples.

MATERIALS PROVIDED
.
Coated Plate Anti-human Factor XI coated 96-well plate 1 plate 4°C CF46a
Standard Lyophilized Human Factor XI standard 1 vial 4°C CF46b
Wash Buffer 10x solution for washing plate 50 mL 4°C CF46c
Primary Antibody Lyophilized Anti-human Factor XI-HRP Conjugate 1 vial 4°C CF46d
SuMATERIALS NEEDED BUT NOT PROVIDED
1. Pipettes covering 0-10 μl and 200-1000 μl and tips
2. 12-channel pipette covering 30-300 μl
3. 1 N H2SO4
4. DI water
5. Microtiter plate spectrophotometer with a 450 nm filter
6. Microtiter plate shaker with uniform horizontally circular movement up to 300 rpm
STORAGE CONDITIONS
1. Store this kit and its components at 4°C until use.
2. The reconstituted Standard and Primary Antibody may be stored at -70°C for later use. DO NOT
freeze/thaw the Standard or Primary Antibody more than once.bstrate TMB Substrate 10 mL 4°C CF46e