Background
Myeloperoxidase (MPO) is a hemoprotein which contains two prosthetic porphyrins that are an essential part of the catalytic cycle. This is an enzyme-linked immunosorbent assay (ELISA) kit. Samples are incubated in the wells of a sectionable microplate,which has been precoated with a monoclonal antibody to MPO. Each kit contains enough reagents to perform 96 MPO assays.
Assay Principle
The MPO-EIA assay system is a sandwich ELISA. Antigen captured by a solid phase monoclonal antibody is detected with an HRP-labeled mouse monoclonal anti-MPO. The HRP substrate TMB is added, and a blue color develops as the reaction proceeds. The reaction is stopped with the addition of the Stop Solution, and the yellow product is measured at 450 nm..jpg)
| References: 1. Klebanoff, Peroxidases in Chemistry and Biology (Everse, Everse, and Grisham eds.), pp.1-35, CRC Press, Boston., 1991. 2. Olsen, and Little, Bio-chem. J. 209:781-787, 1983 |
