TCF/LEF Reporter Kit
Wnt / β-catenin signaling pathway
Catalog #: 60500

BackgroundThe Wnt / β-catenin signaling pathway controls a large and diverse set of cell fate
decisions in embryonic development, adult organ maintenance and disease. Wnt
proteins bind to receptors on the cell surface, initiating a signaling cascade that
leads to stabilization and nuclear translocation of β-catenin. β-catenin then binds
to TCF/LEF transcription factors in the nucleus, leading to transcription and
expression of Wnt-responsive genes.

DescriptionThe TCF/LEF Reporter kit is designed for monitoring the activity of Wnt / β-
catenin signaling pathway in the cultured cells. The kit contains transfection ready
TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive
reporter. This reporter contains a firefly luciferase gene under the control of
multimerized TCF/LEF responsive element located upstream of a minimal
promoter. The TCF/LEF reporter is premixed with constitutively expressing
Renilla luciferase vector that serves as internal control for transfection efficiency.
The kit also includes a non-inducible firefly luciferase vector premixed with
constitutively expressing Renilla luciferase vector as negative control. The noninducible
luciferase vector contains a firefly luciferase gene under the control of a
minimal promoter, without any additional response elements. The negative
control is critical to determining pathway specific effects and background
luciferase activity.

Applications• Monitor Wnt signaling pathway activity.
• Screen activators or inhibitors of Wnt / β-catenin signaling pathway.
• Study effects of RNAi or gene overexpression on the activity of the Wnt
pathway.

Components

These vectors are ready for transient transfection. They are NOT MEANT for
transformation and amplication in bacteria.

Reference:Chen B et al. (2009)Small molecule-mediated disruption of Wnt-dependent
signaling in tissue regeneration and cancer. Nature Chemical Biology
5(2): 100-
107.