PDE4D2 TR-FRET Assay Kit

Catalog # 60707
Size: 96 reactions

DESCRIPTION: Phosphodiesterases (PDEs) play an important role in dynamic
regulation of cAMP and cGMP signaling. PDE4D is a regulator of airway smooth-muscle
contractility, and has been identified as a potential risk predictor for ischemic stroke.
Additionally, PDE4D has been associated with asthma pathophysiology and bone
formation. The PDE4D gene encodes at least 9 different isoforms.
The PDE4D2 TR-FRET Assay Kit is designed for identification of inhibitors of PDE4D2
using TR-FRET (Time Resolved Fluorescence Resonance Energy Transfer) technology.
The assay is based on the generation of FAM-labeled nucleotide monophosphates by
the phosphodiesterase. These phosphate groups bind to terbium-labeled nanoparticles,
resulting in energy transfer from the terbium to the FAM, which emits a fluorescent signal
at 520 nm. The change in fluorescent intensity can be easily measured using a
fluorescence plate reader.

The PDE4D2 TR-FRET Assay Kit comes in a convenient 96-well format, with purified
PDE4D2 enzyme, fluorescently labeled PDE substrate (cAMP), binding agent, and PDE
assay buffer for 100 enzyme reactions. Using this kit, only two simple steps on a
microtiter plate are required for the PDE4D2 activity assay. First, the fluorescent-labeled
cAMP is incubated with a sample containing PDE4D2 for 1 hour. Second, a binding
agent and a terbium donor are added to the reaction mix and incubated for 1 hour. Then,
fluorescence intensity can be measured using a fluorescence reader.

APPLICATIONS: Great for screening small molecular inhibitors for drug discovery and
HTS applications.

STABILITY: 6 months from date of receipt when stored as directed.

COMPONENTS:

REFERENCE(S): Bender. A., et al. Pharmacol. Rev. 2006; 58: 488-520.

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