PCSK9[Biotinylated]-LDLR Binding Assay Kit

Catalog # 72002
Size: 96 reactions

DESCRIPTION: The PCSK9[Biotinylated]-LDLR Binding Assay Kit is designed for
screening and profiling purposes. PCSK9 is known to function as a negative regulator of
hepatic low-density lipoprotein receptors (LDLRs) by binding to the LDLR ectodomain.
The PCSK9[Biotinylated]-LDLR Binding Assay Kit comes in a convenient 96-well format,
with biotin-labeled PCSK9, purified LDLR ectodomain, streptavidin labeled HRP, and
assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of
detection of biotin-labeled PCSK9 by streptavidin-HRP. Only a few simple steps on a
microtiter plate are required for the assay. First, LDLR ectodomain is coated on a 96-well
plate. Next, PCSK9 is incubated with LDLR on the plate. Finally, the plate is treated with
streptavidin-HRP followed by addition of an HRP substrate to produce
chemiluminescence, which can then be measured using a chemiluminescence reader.

COMPONENTS:

APPLICATIONS: Great for screening small molecules and antibodies that inhibit the
binding of PCSK9 to LDLR ectodomain.

STABILITY: One year from date of receipt when stored as directed.

REFERENCES:
1. Chan, J.C., et al. (2009). Proc. Natl Acad. Sci. USA, 106, 9820-9825.
2. Liang, H., et al. (2012). J. Pharmacol. Exp. Ther. 340, 2289-236.

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