PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit
Catalog # 72003
Size: 96 reactions

DESCRIPTION: Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand
attenuates immune responses and is exploited by both tumors and viruses. The PD-
1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and
profiling inhibitors of this signaling. This kit comes in a convenient 96-well format, with
biotin-labeled PD-L1, purified PD-1, streptavidin-labeled HRP, and assay buffer for 100
binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled
PD-L1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required
for the assay. First, PD-1 is coated on a 96-well plate. Next, PD-L1 is incubated with PD-
1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of
an HRP substrate to produce chemiluminescence, which can then be measured using a
chemiluminescence reader.

COMPONENTS:

APPLICATIONS: This kit is useful for screening for inhibitors of PD1 binding to PD-L1.

STABILITY: One year from date of receipt when stored as directed.

REFERENCES:
1. Lin, D., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 3011-3016.
2. Keir, M.E. et al. Annu. Rev. Immunol. 2008, 26: 677-704.

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