PARP6 Chemiluminescent Assay Kit
Catalog # 80556

Size: 32 reactions

DESCRIPTION: The PARP6 Chemiluminescent Activity Assay kit is designed to
measure PARP6 activity for screening and profiling applications. PARP6 is known to
catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The PARP6
assay kit comes in a convenient plate format, with purified PARP6 enzyme, histone
mixture, activated DNA, and PARP assay buffer for 32 enzyme reactions*. The key to
the PARP6 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit,
only three simple steps are required for PARP6 reactions. First, histone proteins are
coated on a microplate. Next, the PARP biotinylated substrate is incubated with an
assay buffer that contains the PARP6 enzyme. Finally, the plate is treated with
streptavidin-HRP followed by addition of the HRP substrate to produce
chemiluminescence that can then be measured using a chemiluminescence reader.
Note: The kit is also suitable for use with cell extracts, but non-specific PARP activity
may be detected.

*The kit includes excess histones, substrate, buffer, DNA and detection reagents for a full 96-well
plate. Additional PARP6 enzyme may be ordered separately if desired (Cat. #80506).

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and screening small molecular
inhibitors for drug discovery and HTS applications.

STABILITY: Up to 1 year when stored as recommended.

REFERENCE: Brown JA, Marala RB. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

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