PARP11 Chemiluminescent Assay Kit
Catalog # 80561

Size: 96 reactions

DESCRIPTION: The PARP11 Chemiluminescent Activity Assay Kit is designed to
measure PARP11 activity for screening and profiling applications. PARP11 is known to
catalyze the NAD-dependent ADP-ribosylation. The PARP11 assay kit comes in a
convenient 96-well format, with purified PARP11 enzyme, histone mixture, and PARP
assay buffer for 100 enzyme reactions. The key to the PARP11 Chemiluminescent
Activity Assay is the biotinylated substrate. With this kit, only three simple steps are
required for PARP11 reactions. First, histone proteins are coated on a 96-well plate.
Next, the biotinylated substrate is incubated with an assay buffer that contains the
PARP11 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition
of the HRP substrate to produce chemiluminescence that can then be measured using a
chemiluminescence reader.

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and screening small molecular
inhibitors for drug discovery and HTS applications.

STABILITY: Up to 1 year when stored as recommended.

REFERENCE:
Brown JA, Marala RB. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

Click here to download product PDF and Protocol