Recombinant Murine LIF
Catalog Number:
250-02
Synonyms:
Leukemia Inhibitory Factor, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor (MLPLI)
Description:
LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Murine LIF is a 19.9 kDa protein containing 180 amino acids residues, including three disulfide bonds.
AA Sequence:
SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF
Source:
E.coli
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
PeproTech's Murine LIF is fully biologically active when compared to standards. The ED50 as determined by the M1 cell differentiation assay is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
Cross Reactivity:
Mouse
Research Interests:
bio-equip.com
PeproTech Inc. 創(chuàng)立于1988年,始終致力于為生命科學(xué)研究開發(fā)重組蛋白及相關(guān)產(chǎn)品,如今已成為能提供多種高質(zhì)量細(xì)胞因子和重組蛋白,相應(yīng)的單克隆、多克隆和生物素標(biāo)記抗體,以及ELISA試劑盒的世界領(lǐng)導(dǎo)者。PeproTech Inc.的產(chǎn)品涵蓋免疫調(diào)節(jié)蛋白、白介素、集落刺激因子、生長因子、化學(xué)趨化因子、干擾素、脂聯(lián)素、成纖維生長因子、轉(zhuǎn)化生長因子、腫瘤壞死因子、神經(jīng)營養(yǎng)素和防御素等諸多生命科學(xué)研究領(lǐng)域中重要的生物活性分子。
為跟蹤當(dāng)今科研發(fā)展、滿足日益增長的需求,PeproTech Inc.研制出無動物成分的重組蛋白(animal-free recombinant proteins)。有效地避免了動物源性污染引發(fā)潛在問題的可能性,從而可以應(yīng)用于臨床前研究和細(xì)胞治療。此外,PeproTech Inc.最新還開發(fā)出無動物成分的培養(yǎng)基添加物試劑盒(animal-free Media Supplement Kits)。這些試劑盒經(jīng)改良設(shè)計,其中的無血清組分對多種哺乳動物細(xì)胞的生長有更好的維持能力。
PeproTech Inc.的產(chǎn)品自2000年進(jìn)入中國市場以來,以其優(yōu)良的品質(zhì)和合理的價格迅速得到國內(nèi)各重點(diǎn)研究和實(shí)驗(yàn)機(jī)構(gòu)的認(rèn)可和青睞。鑒于中國經(jīng)濟(jì)發(fā)展的良好態(tài)勢和中國生物醫(yī)藥研發(fā)投入的巨額增長,PeproTech Inc.于2009年10月在中國蘇州設(shè)立代表機(jī)構(gòu),意為中國的廣大用戶提供更好的支持和服務(wù),并助中國的科研和產(chǎn)業(yè)開發(fā)一臂之力。