Chromatrap Enymatic ChIP qPCR 蛋白 A
Product Code : 500166
套件內容物:Chromatrap® 蛋白 A離心柱(24)、柱調控緩沖液、清洗緩沖液1、清洗緩沖液2、清洗緩沖液3、1.3M甘氨酸、裂解緩沖液、消化緩沖液、酶終止液、0.1M氫氧化鈉、5M氯化鈉、1M碳酸氫鈉、低滲緩沖液、蛋白酶抑制劑(PIC)、蛋白酶K終止液、蛋白酶K和1.5 ml采集管。
酶促 ChIP qPCR
用于qPCR的Chromatrap® Enzymatic ChIP套件可通過使用其獨有的專利技術簡化流程迅速改善ChIP,確保用于qPCR分析的染色質制備可在5小時內完成。
為避免使用磁珠或瓊脂糖珠,非特異性背景被消除,同時減少人工操作錯誤和后續樣品損失。優化洗脫緩沖液化學分析允許樣品直接在qPCR中分析,而無需進行DNA清理,從而進一步降低樣品損失。
對于不能使用超聲儀剪切ChIP實驗所需DNA,Enzymatic ChIP套件是完美替代品。ChIP qPCR實驗是一項耗時短且便捷的低染色質負荷(40μls)實驗,懸浮液體積小,qPCR前未執行DNA清理步驟。該套件被設計僅用作如qPCR的下游過程,并具有適合低染色質負荷(1µg-10µg)的寬動態范圍。
★ 為1000ng樣品尺寸進行的優化可使小樣品尺寸獲得更好的結果,并提供更大的靈活性,因而可針對1件樣品進行多次IP實驗。
★ 該套件含有基于蛋白A或蛋白G的足夠離心柱、緩沖液和試劑,可執行24次染色質免疫共沉淀實驗以及染色質(10次制備)的酶切。
★ 高通量樣式還允許多種樣品類型、抗體和基因打靶并行接受試驗。
包括陽性和陰性抗體以及引物的高級套件可提供給您令人滿意的染色質制備。
bio-equip.com
在中國的代理商
北京亙辰科技有限公司(Beijing Genechain Technology Ltd)
電話: 010 - 6291 7082 手機:138 1083 8040
Porvair Sciences, specialists in the manufacture of microplate products, serve Life Sciences, Biotechnology, R&D and Molecular Biology with microplate solutions for all applications, from sample preparation to high throughput screening via our global distributor network.
Our range includes vacuum manifolds, sealers, evaporators and microtiter plates in all popular styles; deep well and shallow well storage plates, assay plates, luciferase reporter gene plates and liquid handling reagent reservoirs. We also provide custom microplate products for life science research.
Our vacuum manifolds, essential for 96-well SPE sample preparation, are designed to work with most popular filter plates, including Waters, Millipore, Qiagen, Whatman, GE Healthcare, Varian, Biotage and, of course, Porvair.
For further information, including custom built microplates, reagent reservoirs, vacuum manifold design or microplate handling equipment, please contact us: Tel: +44 1978 666240; Email: int.sales@porvair-sciences.com.