由于環(huán)境因素和細胞內(nèi)的正常代謝過程造成的DNA損傷，每個細胞每天都會發(fā)生1,000到1,000,000個。雖然這些只占人類基因組約60億個堿基中的一小部分，但如果關(guān)鍵基因損傷未及時修復(fù)，可能會阻礙細胞的正常生理功能，進而增加癌變可能。彗星實驗，或稱單細胞凝膠電泳（Single cell gel electrophoresis，SCGE），是一種測量單個細胞DNA損傷的常用技術(shù)。其原理很簡單，即在電泳場中，將受損細胞DNA（包含片段和鏈斷裂）與完整的DNA分離，通過顯微鏡可觀察到損傷細胞呈現(xiàn)出典型的彗星狀尾巴，然后通過測量計算彗尾大小對比出細胞DNA損傷的程度。因為彗星實驗的特點，該方法幾乎被用來評估任何類型的真核細胞的 DNA 修復(fù)能力，包括雙、單鏈斷裂的不同的 DNA 損傷情況。是一種能快速、大通量檢測真核細胞DNA損傷進而判別遺傳毒性的技術(shù)。

彗星實驗結(jié)果圖

彗星實驗原理雖簡單，但操作繁瑣，需要豐富的實驗經(jīng)驗和技巧，尤其常常出現(xiàn)的“脫膠”問題，困擾了許多科研人員。除此之外，有時為了跑出完美的“彗星”圖案放在paper里，還需要重復(fù)做許多次實驗，費時費力。為了解決上述問題，我們推薦CellBiolabs的OxiSelectTMComet Assay Kit即彗星實驗試劑盒來檢測細胞的DNA損傷。該試劑盒不僅能讓彗星實驗化繁為簡，還有兩種不同規(guī)格（3孔和96孔）的細胞電泳凝膠板供選擇，讓少量樣本和大量樣本的DNA損傷檢測通通輕松hold住。用該試劑盒做彗星實驗流程如下圖。

除了操作簡便，OxiSelectTMComet Assay Kit還有以下優(yōu)點：

1)      適用于各種DNA損傷檢測，是一款非常好用的DNA損傷檢測篩選工具；

2)      試劑盒中的載玻片經(jīng)過特殊處理以粘附低熔點瓊脂糖，避免“脫膠”問題出現(xiàn)；

3)      采用特殊的DNA熒光染料，能有效降低背景干擾，更加方便讀取實驗結(jié)果。

彗星實驗試劑盒信息：

為了滿足客戶更多樣的實驗需求，彗星實驗試劑盒內(nèi)特殊處理電泳載玻片還可以單獨購買，詳情如下：

產(chǎn)品部分發(fā)表文獻：

1. Maiuri, T. et al. (2016). Huntingtin is a scaffolding protein in the ATM oxidative DNA damage response complex. Hum. Mol. Genet. doi:10.1093/hmg/ddw395.
2. Irianto, J. et al. (2016). Nuclear constriction segregates mobile nuclear proteins away from chromatin. Mol. Bio. Cell doi:10.1091/mbc.E16-06-0428.
3. Andronescu, E. et al. (2016). Nanomaterials for medical applications: Benefits and risks. J Nanomater. doi:10.1155/2016/8284319.
4. Liu, Z. et al. (2016). Canonical microRNAs enable differentiation, protect against DNA damage, and promote cholesterol biosynthesis in neural stem cells. Stem Cells and Dev. doi:10.1089/scd.2016.0259.
5. Dai, C. et al. (2016). Curcumin ameliorates furazolidone-induced DNA damage and apoptosis in human hepatocyte L02 cells by inhibiting ROS production and mitochondrial pathway. Molecules. 21:1061.
6. Beegle, J. R. et al. (2016). Preclinical evaluation of mesenchymal stem cells overexpressing VEGF to treat critical limb ischemia. Mol Ther Methods Clin Dev.doi:10.1038/mtm.2016.53.
7. Suzuki, Y. et al. (2016). Pharmacodynamics of anti-inflammatory drugs–intranasal corticosteroid and dna damage. Pharmacodynamics of anti-inflammatory drugs. 117-126.
8. Zhang, J. et al. (2016). Inhibition of Glucose-6-Phosphate Dehydrogenase could Enhance 1, 4-Benzoquinone-induced Oxidative Damage in K562 Cells. Oxid Med Cell Longev. doi:10.1155/2016/3912515.
9. Jang, J. H. et al. (2016). APO-9′-fucoxanthinone extracted from undariopsis peteseniana protects oxidative stress-mediated apoptosis in cigarette smoke-exposed human airway epithelial cells. Mar Drugs. doi:10.3390/md14070140.
10. Ebeid, S. A. et al. (2016). Assessment of the radioprotective effect of propolis in breast cancer patients undergoing radiotherapy. New perspective for an old honey bee product. J Radiat Res Appl Sci. doi:10.1016/j.jrras.2016.06.001.
11. Wang, H, & Kim, N. H. (2016). CDK2 is required for the DNA damage response during porcine early embryonic development. Biol Reprod. doi:10.1095/biolreprod.116.140244.
12. Zhao, X. et al. (2016). Dioscin induces apoptosis in human cervical carcinoma HeLa and SiHa cells through ROS-mediated DNA damage and the mitochondrial signaling pathway. Molecules. doi:10.3390/molecules21060730.
13. Chen, X. et al. (2016). Zidovudine, abacavir and lamivudine increase the radiosensitivity of human esophageal squamous cancer cell lines. Oncol Rep. 36:239-246.
14. Coleman, J. et al. (2016). Detecting Apoptosis, Autophagy, and Necrosis. Apoptosis Methods in Toxicology . doi:10.1007/978-1-4939-3588-8_5.
15. Ding, Y. et al. (2016). Induction of ROS overload by alantolactone prompts oxidative DNA damage and apoptosis in colorectal cancer cells. Int J Mol Sci. doi:10.3390/ijms17040558.
16. Cui, F. M. et al. (2016). The role of miR-34a in tritiated water toxicity in human umbilical vein endothelial cells. Dose-Response. doi:10.1177/1559325816638585.
17. Laks, D. R. et al. (2016). Inhibition of nucleotide synthesis targets brain tumor stem cells in a subset of glioblastoma. Mol Cancer Ther. doi:10.1158/1535-7163.MCT-15-0982.
18. Abubakar, I. B. et al. (2016). Synergistic cytotoxic effects of combined δ-tocotrienol and jerantinine B on human brain and colon cancers. J Ethnopharmacol. doi:10.1016/j.jep.2016.03.004.
19. Hofstetter, C. et al. (2016). Inhibition of KDM6 activity during murine ESC differentiation induces DNA damage.J Cell Sci. 129:788-803.
20. Si, L. et al. (2016). Dioscin suppresses human laryngeal cancer cells growth via induction of cell-cycle arrest and MAPK-mediated mitochondrial-derived apoptosis and inhibition of tumor invasion. Eur J Pharmacol. doi:10.1016/j.ejphar.2016.02.009.
21. Qu, L. et al. (2016). Corosolic acid analogue, a natural triterpenoid saponin, induces apoptosis on human hepatocarcinoma cells through mitochondrial pathway in vitro. Pharm Biol. doi:10.3109/13880209.2015.1104699.
22. Singh, A. K. et al. (2015). Parental age affects somatic mutation rates in the progeny of flowering plants. Plant Physiol. doi:10.1104/pp.15.00291.
23. Yuan, L. et al. (2015). Serum collected from fruit and vegetable juice treated rats antagonizing H2O2-induced oxidative damage in PC12 cells. J Funct Foods. 20:496-505.
24. Ramy, N. et al. (2015). Jaundice, phototherapy and DNA damage in full-term neonates.  J Perinatol.  doi:10.1038/jp.2015.166.
25. Wu, C. F. et al. (2015). Anticancer activity of cryptotanshinone on acute lymphoblastic leukemia cells. Arch Toxicol. doi:10.1007/s00204-015-1616-4.
26. Kim, M. J. et al. (2015). Antibacterial effect and mechanism of high-intensity 405±5nm light emitting diode on Bacillus cereus, Listeria monocytogenes, and Staphylococcus aureus under refrigerated condition. J Photochem Photobiol B. 153:33-39.
27. Wang, X. et al. (2015). Enhancement of arabinocytosine (AraC) toxicity to AML cells by a differentiation agent combination. J Steroid Biochem Mol Biol.  doi:10.1016/j.jsbmb.2015.08.023.
28. Sun, X. et al. (2015). Electrochemical detection of 8-hydroxy-2′-deoxyguanosine as a biomarker for oxidative DNA damage in HEK293 cells exposed to 3-chloro-1, 2-propanediol. Anal Methods.doi:10.1039/C5AY01246E.
29. Neumann, J. et al. (2015). Mangrove dolabrane‐type of diterpenes tagalsins suppresses tumor growth via ROS‐mediated apoptosis and ATM/ATR–Chk1/Chk2‐regulated cell cycle arrest. Int J Cancer.  doi: 10.1002/ijc.29629.
30. Singh, A. K. et al. (2015). Parental age affects somatic mutation rates in the progeny of flowering plants. Plant Physiol. 168:247-257.
31. Hou, W. et al. (2015). The protecting effect of deoxyschisandrin and schisandrin B on HaCaT cells against UVB-induced damage. PLoS One. 10:e0127177.
32. Kim, M. J. et al. (2015). Inactivation by 405±5 nm light emitting diode on Escherichia coli O157: H7, Salmonella Typhimurium, and Shigella sonnei under refrigerated condition might be due to the loss of membrane integrity. Food Control. doi:10.1016/j.foodcont.2015.05.012.
33. Haeger, S. M. et al. (2015). Smad4 loss promotes lung cancer formation but increases sensitivity to DNA topoisomerase inhibitors. Oncogene.  doi: 10.1038/onc.2015.112.
34. Ong, J. Y. et al. (2015). 2-Methoxy-1, 4-naphthoquinone (MNQ) induces apoptosis of A549 lung adenocarcinoma cells via oxidation-triggered JNK and p38 MAPK signaling pathways. Life Sci. doi: 10.1016/j.lfs.2015.03.019.
35. Prasad, M. A. et al. (2015). Ebf1 heterozygosity results in increased DNA damage in pro-B cells and their synergistic transformation by Pax5 haploinsufficiency. Blood.  doi: 10.1182/blood-2014-12-617282.
36. Obstoy, B. et al. (2015). Safety and performance analysis of acriflavine and methylene blue for in vivo imaging of precancerous lesions using fibered confocal fluorescence microscopy (FCFM): an experimental study. BMC Pulm Med. 15:30.
37. Xiong, J. et al. (2015). Stemness factor Sall4 is required for DNA damage response in embryonic stem cells.J Cell Biol. 208:513-520.
38. Hu, Y. et al. (2015). Bile acids regulate nuclear receptor (nur77) expression and intracellular location to control proliferation and apoptosis. Mol Cancer Res. 13:291-292.
39. Gong, L. et al. (2015). p53 isoform Δ113p53/Δ133p53 promotes DNA double-strand break repair to protect cell from death and senescence in response to DNA damage. Cell Res. 25:351-369. 
40. Jin, L. et al. (2015). Association between oxidative DNA damage and the expression of 8-oxoguanine DNA glycosylase 1 in lung epithelial cells of neonatal rats exposed to hyperoxia. Mol Med Rep. doi: 10.3892/mmr.2015.3339.