描述
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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Platinum SYBR Green qPCR SuperMix-UDG and added additional capabilities for your gene expression analysis.
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qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA. ROX reference dye is included in the mix for maximum convenience. These qPCR SuperMixes include:
–Platinum®
Taq DNA Polymerase: activates faster and more completely than other hot-start enzymes to deliver more sensitive amplification (Figure 1)
–Uracil-DNA Glycosylase (UDG): prevents carryover contamination in conjunction with dUTP (included) for improved specificity
–ROX reference dye premixed at optimal concentration for ABI instruments
qPCR SuperMixes with ROX are available in two formats:
–Platinum® SYBR® Green qPCR SuperMix-UDG with ROX provides a sensitive, cost-effective system for real-time PCR detection with SYBR® Green I dye (Figure 2)
–Platinum® qPCR SuperMix-UDG with ROX offers superior performance with either fluorogenic primers (e.g., LUX™ Primers) or probe-based detection systems (e.g., TaqMan®)
Contents and Storage:
Platinum® SYBR® Green qPCR SuperMix-UDG with ROX includes a 2X SuperMix with SYBR® Green I and ROX Reference Dyes, and 50 mM MgCl2. Platinum® qPCR SuperMix-UDG with ROX includes a 2X SuperMix with ROX Reference Dye, and 50 mM MgCl2. Store all components at -20°C. Guaranteed stable for 6 months when properly stored.
規格
Detection Method: |
SYBR |
For Use With (Equipment): |
7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System |
Reaction Speed: |
Standard |
PCR Method: |
qPCR |
Sample Type (General): |
DNA |
Product Line: |
Platinum®, SYBR® |
Product Size: |
500 reactions |
Sample Type (Specific): |
DNA (Genomic), cDNA |
Shipping Condition: |
Dry Ice |
內容及儲存
Contains 2X Platinum SYBR Green qPCR SuperMix-UDG with ROX (12.5 mL) and 50 mM Magnesium Chloride (2 × 1 mL).
Contains sufficient reagents for 500 reactions of 50 µL each. Store in freezer (-5 to -30°C).
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