產品貨號：K-URAMR 

品牌：Megazyme

產品品名：尿素/氨[快速]檢測試劑盒

英文品名：Urea/Ammonia (Rapid) Assay Kit

規格型號：100 assays - 50 of each component 

For the specific and rapid assay of urea and ammonia in water,beverages, milk and food products.

INTRODUCTION:

Urea and ammonia are widely occurring natural compounds. As urea is themost abundant organic solute in urine, and ammonia is produced as aconsequence of microbial protein catabolism, these analytes serve asreliable quality indicators for food products such as fruit juice, milk,cheese, meat and seafood. Ammonium carbonate is used as a leaven inbaked goods such as quick breads, cookies and muffins. Unlike some otherkits, this kit benefits from the use of a glutamate dehydrogenase thatis not inhibited by tannins found in, for example, grape juice and wine.In the wine industry, ammonia determination is important in thecalculation of yeast available nitrogen (YAN). YAN is comprised of threehighly variable components, free ammonium ions, primary amino nitrogen(from free amino acids) and the contribution from the sidechain ofL-arginine1. For the most accurate determination of YAN, all threecomponents should be quantified, and this is possible using Megazyme’s
L-Arginine/Urea/Ammonia Kit (K-LARGE) and NOPA Kit
(K-PANOPA). Urea determination can be important in preventing theformation of the known carcinogen ethyl carbamate (EC) in finished wine.

KITS:

Kits suitable for performing 50 assays (of each) are available fromMegazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (18 mL, pH 8.0) plus 2-oxoglutarate and
sodium
azide (0.02% w/v) as a preservative.
Stable for
> 2 years at 4°C.
Bottle 2: NADPH. Lyophilised powder.
Stable for > 5 years at -20°C.
Bottle 3: Glutamate dehydrogenase suspension (1.1 mL).
Stable for
> 2 years at 4°C.
Bottle 4: Urease solution (2.7 mL).
Stable for
> 2 years at -20°C.
Bottle 5: Urea control powder (~ 2 g).
Stable for
> 2 years at room temperature.


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