產品貨號：K-FUCOSE 

品牌：Megazyme

產品品名：L-海藻糖檢測試劑盒

英文品名：L-Fucose Assay Kit

規格型號：100 assays (manual) / 1000 assays (microplate) / 1020 assays (auto-analyser) 

A simple, rapid and reliable method, for the measurement of L-Fucose inplant extracts, biological samples and other materials. This kit can beused in the measurement of α-fucosidases that do not act on chromogenic substrates. 


INTRODUCTION:

L-Fucose is a 6-carbon deoxyhexose and exists in nature in variousbiological niches. A major natural source of L-fucose is the brown algalpolysaccharide fucoidan.
L-Fucose is a minor component in plant cell wall polysaccharides,specifically, rhamnogalacturonan, xyloglucan and also arabinogalactanproteins that are involved in plant cell elongation.
In glycoproteins L-fucose is a key component of the oligosaccharidemoiety and is attached via α-1,(2, 3, 4 or 6) linkages. The variation ofthe oligosaccharide structure is known to dictate biological function ofglycoproteins and, as such, L-fucose has been indicated as a potentialbiological marker for various disease states such as breast cancer.L-fucose is a common component of human milk oligosaccharides (HMO) suchas 2’-fucosyl-lactose. In addition to the role as a prebiotic, HMOs areknown to impart immunological functions to breast-fed infants such aspreventing microbial adhesion to the intestinal mucosal membrane.
This kit (K-FUCOSE) provides a rapid and reliable method, suitable forthe measurement of L-fucose in plant extracts, biological samples andother materials. It can also be used in the measurement ofα-L-fucosidases that do not act on chromogenic substrates.

KITS:

Kits suitable for performing 100 assays in manual format (or 1000assays in microplate format or 1020 assays in auto-analyser format) areavailable from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (44 mL; pH 9.5) plus sodium azide
(0.02% w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 2: NADP+.
Freeze dried powder.
Stable for > 5 years at -20°C.
Bottle 3: L-Fucose dehydrogenase suspension (2.2 mL).
Stable for > 2 years at 4°C.
Bottle 4: L-Fucose (5 mL, 0.5 mg/mL).
Stable for > 2 years at 4°C.

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