產品貨號：K-GCROLGK 

品牌：Megazyme

產品品名：甘油（葡萄糖激酶法快速）檢測試劑盒

英文品名：Glycerol GK Assay Kit

規格型號：70 assays (manual) /700 assays (microplate) / 600 assays(auto-analyser)  

A simple, reliable and accurate method for the measurement of glycerolin beverages, foodstuffs and other material. Based on use ofADP-glucokinase and increase inabsorbance on conversion of NAD+ to NADH.

INTRODUCTION:

In the food industry, glycerol is an important moistening agent
for baked goods. It is also added to candies and icings to prevent
crystallisation and as a solvent for food colours and carrier for
extracts and flavouring agents. As a product of fermentation, glycerol
is monitored in the beer and wine industries, where it occurs at
concentrations of approx. 1% (v/v), and is an indicator of product
quality. The smoothness of lotions, creams and toothpaste is due
to the incorporation of glycerol. Due to its hygroscopic properties,
glycerol is sprayed on pre-processed tobacco to prevent crumbling.
This kit benefits from the presence of ADP-glucokinase which permits
the detection and measurement of glycerol from an increase in
absorbance.

KITS:

Kits suitable for performing 70 assays in manual format (or 600
assays in auto-analyser format or 700 assays in microplate format) are
available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (20 mL, pH 7.4).
Stable for > 2 years at 4°C.
Bottle 2: (x2) NAD+, ATP and D-glucose.
Freeze dried powder.
Stable for > 5 years at -20°C.
Bottle 3: ADP-glucokinase plus glucose-6-phosphate
dehydrogenase suspension, 1.5 mL.
Stable for > 2 years at 4°C.
Bottle 4: Glycerokinase suspension (1.5 mL).
Stable for > 2 years at 4°C.
Bottle 5: Glycerol standard solution (5 mL, 0.20 mg/mL)
in 0.02% (w/v) sodium azide.
Stable for > 2 years at 4°C.

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