產品貨號：K-GLNAM 

品牌：Megazyme

產品品名：L-谷氨酸 氨快速檢測試劑盒

英文品名：L-Glutamine / Ammonia (Rapid) Assay Kit

規格型號：100 assays – 50 of each component

Novel method for the specific, convenient, cost effective and rapidassay of L-glutamine and ammonia in culture media/supernatants and other materials.

INTRODUCTION:

L-Glutamine is an essential component of certain cell culture media.However, the incorporation of this amino acid into growth media presentstwo major problems; firstly, L-glutamine is labile, and spontaneouslybreaks down to L-glutamate and free ammonium ions, and secondly, thereleased ammonium ions are very toxic to the cells. To overcome theseissues, either pre-formulated growth media are used strictly withintheir recommended shelf-lives or L-glutamine is added just before use.In either case, monitoring of L-glutamine and ammonia is frequentlyperformed both prior to, and during culturing.
Individual test kits do exist for L-glutamine and ammonia, but theseproducts can be very slow, owing to either the limiting amounts ornature of the enzymes employed. Megazyme thus developed this kit(K-GLNAM), based on advanced recombinant enzymes, that is both veryrapid (~ 15 min) and also measures both analytes in a very simpleformat. As the kit is based on quantitative endpoint readings, acalibration curve is not required, saving more time and expense. Bothmanual (see page 5, “A”) and microplate (see page 8, “B”) formats aredescribed. Other rapid cell culture test kits are also available fromMegazyme for ammonia (K-AMIAR), L-asparagine / ammonia (K-ASNAM),D-glucose (K-GLUHKR or K-GLUC) and L-lactic acid (K-LATE).
This kit is also suitable for the analysis of L-glutamine in a widerange of other samples, such as dietary supplements and vegetables.

KITS:

Kits suitable for performing 50 assays of both ammonia and
L-glutamine are available from Megazyme. The kits contain the fullassay method plus:
Bottle 1: Buffer (11 mL, pH 4.9) plus sodium azide
(0.02% w/v) as
a preservative Stable for > 2 years at 4°C.
Bottle 2: (x2) Buffer (25.5 mL, pH 8.0) plus sodium azide
(0.02% w/v) as
a preservative.
Stable for
> 2 years at 4°C.
Bottle 3: (x2) NADPH. Lyophilised powder.
Stable for > 5 years at -20°C.
Bottle 4: Glutaminase suspension (1.1 mL).
Stable for
> 2 years at 4°C.
Bottle 5: Glutamate dehydrogenase (2.2 mL).
Stable for
> 2 years at 4°C.
Bottle 6: Ammonia standard solution (5 mL, 0.04 mg/mL) in 0.02% (w/v)sodium azide.
Stable for
> 2 years at 4°C.
Bottle 7: L-Glutamine control powder (~ 2 g).
Stable for
> 2 years at 4°C.

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