產品貨號：K-GLUM

品牌：Megazyme

產品品名：葡甘露聚糖檢測試劑盒

英文品名：DGlucomannan Assay Kit

規格型號：50 Assays per kit  

For the measurement of Glucomannan in plant products and food.

INTRODUCTION:

Glucomannan is a linear polysaccharide comprising 1,4-β-linked
D-glucosyl and D-mannosyl residues, where the glucose : mannose(Glc:Man) ratio varies considerably. Konjac (Amorphophallus konjac)glucomannan has a Glc:Man ratio of approx. 2:3, Aloe vera glucomannan aratio of 1:2, and salep (Orchis mureo) glucomannan has a ratio ofapproximately 1:4. All of the polysaccharides are acetylated, whichimparts water solubility. Glucomannans are major cell-wall reservecarbohydrates in tubers of several plant genera. At certain stages inthe development of the plants, the glucomannan is degraded and serves asan energy source. The only industrially exploited glucomannan is konjac.Like carob galactomannan, this polysaccharide interacts with agar,carrageenan and xanthan to form solutions of increased viscosity orgels. This interaction is exploited in the pet-food industry in canneddog food. Recently, glucomannan has also been used in jelly sweets. Inthis application, the glucomannan has been found to be dangerous,leading to choking deaths of some consumers. This has led to a ban ofthese types of sweets in Europe and America, and a need for an accurateanalytical procedure for glucomannan analysis.

KITS:

Kits suitable for performing 50 determinations of glucomannan areavailable from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (25 mL, pH 4.5) plus sodium azide
(0.02% w/v) as
a preservative.
Stable for > 2 years at 4°C.
Bottle 2: Buffer (25 mL, pH 7.6) plus magnesium chloride and sodiumazide (0.02% w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 3: NADP+ plus ATP.
Stable for
> 5 years at -20°C.
Bottle 4: β-Mannanase suspension (1.2 mL).
Stable for
> 2 years at 4°C.
Bottle 5: β-Glucosidase plus β-mannosidase suspension, 1.1 mL.
Stable for
> 2 years at 4°C.
Bottle 6: Hexokinase plus glucose-6-phosphate dehydrogenase suspension,1.1 mL.
Stable for
> 2 years at 4°C.
Bottle 7: Phosphoglucose isomerase plus phosphomannose isomerasesuspension, 1.1 mL.
Stable for
> 2 years at 4°C.
Bottle 8: D-Glucose plus D-mannose standard solution
(0.2 mg/mL
of each).
Stable for > 2 years at 4°C.

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