產品貨號：K-AZOWAX  

品牌：Megazyme

產品品名：木聚糖酶檢測試劑盒

英文品名：Aspartame Assay Kit

規格型號： 200 assays per kit 

For the measurement of endo-1,4-?-D-xylanase in enzyme preparations,bread improver mixtures and animal feeds. Containing Azo-wheatarabinoxylan and a Trichoderma sp. xylanase control.

INTRODUCTION:

Arabinoxylan is the major endosperm cell-wall polysaccharide of wheatand rye and is found in significant proportions in most cereal solutionsand slurries of high viscosity and, in animal nutrition, it reduces therate of nutrient absorption from the gut.
endo-β-D-Xylanase (xylanase) is added to feeds to catalyse
depolymerisation of this polysaccharide. It can be demonstrated thatendo-cleavage by xylanase of just one bond per thousand in thearabinoxylan backbone can significantly remove viscosity properties.
Of the carbohydrase enzymes used as feed supplements, one of the mostdifficult to measure has been xylanase. These problems are attributed toseveral factors, including the low levels of enzyme
added to the feed, inactivation of enzyme during pelleting, binding
of the enzyme to feed components and the presence of specific xylanase inhibitors.
The only biochemical methods which are sufficiently sensitive, specificand robust to measure xylanase in feeds are viscometric assays and thoseemploying dyed xylan or arabinoxylan polysaccharides.
Viscometric assays are tedious, whereas assays employing dyed xylansubstrates are rapid, reproducible and simple to perform. We recommendthe use of either Xylazyme AX tablets or Azo-Wheat Arabinoxylan(Azo-WAX). Xylazyme AX based assays are about 5-fold more sensitive thanassays employing Azo-WAX. However, this latter substrate does havesufficient sensitivity in most applications, and results are slightlymore reproducible than with Xylazyme AX.
It is generally accepted that xylanase enzymes which are best suited tofeed applications have optimal activity at pH 6.0. Consequently, theseenzymes are generally assayed at this pH in 100 mM sodium phosphatebuffer. In recovery experiments, however, we found that sodium phosphatebuffer extracts only a small proportion
(< 20%) of the amount of enzyme added to the feed. Thus a wide rangeof alternative extractants and extraction conditions have beenevaluated. For feeds containing Trichoderma sp. xylanases, the best andmost consistent results have been obtained using 100 mM acetic acid or100 mM sodium acetate buffer (pH 4.7) at room temperature. Optimalextraction of Humicola sp. xylanases was achieved with a buffercontaining 100 mM MES buffer (pH 6.0) and 1% w/v sodium dodecyl sulphate (SDS).

KITS:

Kits containing the required reagents to measure xylanase in animalfeeds are available from Megazyme. These kits contain:
1. Azo-wheat arabinoxylan (100 mL, 1% w/v).
2. Trichoderma sp. control xylanase [3700 milli-Units (mU)/mL; pH 6.0and 40°C] in 50% glycerol.

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