產(chǎn)品貨號：K-ACET

品牌：Megazyme

產(chǎn)品品名：乙酸檢測試劑盒

英文品名：Acetic Acid (ACS Manual Format) assay Kit

規(guī)格型號：53 Assays per Kit

A simple method for the rapid and reliable measurement of aceticacid/acetate in foods, beverages and other materials. 


INTRODUCTION:

Acetic acid (acetate) occurs in a wide range of foods and beverages,

and in many other materials such as paper, pharmaceuticals and

industrial products. In the wine industry it is one of the most

important quality parameters and is measured throughout the entire

vinification process. This advanced kit (K-ACET) benefits from

greatly increased shelf life as the acetyl-coenzyme A synthetase

(ACS) is supplied as a highly stable ready-to-use ammonium sulphate

suspension, rather than as a lyophilised powder. Polyvinylpyrollidone

(PVP) has also been incorporated into the assay to prevent

interference from particular tannins found especially in red wines.

This kit is recommended for manual analysis, while two additional kits

have been optimised for auto-analysis applications; K-ACETAF has

extended calibration linearity up to 30 μg/mL (in the final assay), and

also benefits from a stabilisation system that significantly reduces the

rate of deterioration (increase in absorbance) of prepared reagent,

a problem exhibited by some other products; K-ACETAK is a new

assay format based on acetate kinase. As an indicator reaction is

not involved in this case, a change in absorbance stoichiometric with

the amount of acetic acid is achieved, resulting in excellent linear

calibration (R2 > 0.999).

KITS:

Kits suitable for performing 53 determinations are available from

Megazyme. The kits contain the full assay method plus:

Bottle 1: Buffer (30 mL, pH 8.4) plus L-malic acid and sodium

azide (0.02 % w/v) as a preservative.

Stable for > 2 years at 4°C.

Bottle 2: (x2) NAD+ plus ATP, PVP and CoA.

Freeze dried powder.

Stable for > 5 years at -20°C.

Bottle 3: L-Malate dehydrogenase plus citrate synthase

suspension, 1.1 mL.

Stable for > 2 years at 4°C.

Bottle 4: Acetyl-coenzyme A synthetase suspension (1.1 mL).

Stable for > 2 years at 4°C.

Bottle 5: Acetic acid standard solution (5 mL, 0.10 mg/mL).

Stable for > 2 years at 4°C.

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