Recombinant Human Heparanase 1 (HPA1) –
Western Blot Control

Catalog No. INS-26-5-0000-10
BACKGROUND
Heparanase is an endo β-D-glucuronidase, which degrades heparan
sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the
extracellular matrix. Heparanase plays an important role in ECM
degradation, facilitating the migration and extravasation of tumor
cells and inflammatory leukocytes (1,2,3). Upon degradation,
heparanase releases growth factors and cytokines that stimulate cell
proliferation and chemotaxis (4,5). Heparanase is a heterodimer
comprised of a 50 kDa subunit harboring the active site and a 8 kDa
subunit. It is produced as a latent 65 kDa precursor and
proteolytically processed to its active form (1,6). Heparanase is
highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets
and in human placenta. Human heparanase was found to be
upregulated in various types of primary tumors, correlating in some
cases with increased tumor invasiveness and vascularity and with
poor prospective survival (7,8).
PRODUCT DESCRIPTION
Recombinant Heparanase protein HPA1 is produced in CHO cells.
The protein is purified by several orthogonal chromatography steps.
QUANTITY AND FORMULATION
Concentration: 1μg /ml
Content: 100 ng
Buffer: LDS-PAGE buffer
[140 mM Tris buffer pH 8.5, 10% Glycerol, 2% LDS, 0.015%
EDTA, 1.88% (v/v) of 1% Serva Blue G250 and 0.625% (v/v) of 1%
Phenol red]
APPLICATION
Positive control for western blot analysis.
USE
Use 20 μl of recombinant human heparanase 1 (HPA1) per lane, as a
control for using monoclonal anti HPA 1 clone HP3/17 antibodies
(Cat. No.: Ins-AB-04001) or polyclonal rabbit anti HPA1 antibody
(Cat. No.: Ins-AB-04002).
STORAGE
Store at –200C, avoid repeated freeze-thaw cycles.
RESEARCH USE
For in vitro research use only. Not for use in diagnostic procedures.

http://www.insight.co.il/files/wb%20control_2006.pdf