Background
This rat plasminogen activator inhibitor type 1 (PAI-1) total antigen assay is intended for the quantitative determination of total PAI-1 in biological fluids.
Plasminogen activator inhibitor-1 (PAI-1) is a central regulator of the blood fibrinolytic system [1]. Clinical studies have indicated that increased PAI-1 levels increase the risk for thrombosis, whereas decreased levels may cause recurrent bleeding (2).
Assay Principles
Rat PAI-1 will bind to the capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will react with the capture antibody on the plate. Any unbound PAI-1 is washed away and an anti PAI-1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the total PAI-1 present in the samples, is then reacted with a secondary antibody. Following an additional washing step, TMB is then used for color development at 450 nm. The amount of color development is directly proportional to the concentration of total PAI-1 in the sample.
Typical Standard Curve
References
1. Eitzman, D.T., et al.: (2000) Blood; 95(2): 577-580
2. Kawasaki, T., et al.: (2000) Blood; 96(1): 153-160
3. Ngo, T.H., et al.: (1998) Thromb Haemostas; 79(4): 808-812
4. Schafer, K., et al.: (2001) FASEB J; 15: 1840-1842
5. Samad, F., et al.: (1996) Proc Natl Acad Sci USA; 96(12): 6902-6907
6. Eitzman, D.T., et al.: (2000) Blood; 95(2): 577-580
7. Seki, T., et al.: (1996) Thromb Haemostas; 75(5): 801-807
