PDE1B Cell-Based Reporter Assay Kit
Catalog #: 60601

Background: 
PDE1B plays a key role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE1B hydrolyzes both cAMP and cGMP to nucleoside 5
-monophosphate and prefers cGMP at low substrate levels.

Description: 

The PDE1B Cell-Based Reporter Assay Kit is designed for screening inhibitors of PDE1B in cultured cells. The kit is also ideal for monitoring PDE1B/ cGMP/ Ca2+ channel signaling pathway activity in vivo. It is useful in determining transfection efficiency. The kit includes a recombinant HEK293 cell line that stably expresses human PDE1B, atrial natriuretic peptide (ANP), mammalian expression constructs for ANP receptor A, CNGA2, and aequorin, and buffer for the assay.

Components:

*The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria. 

References:
1. Yu, J. et al. (1997). “Identification and Characterization of a Human Calmodulin-Stimulated Phosphodiesterase PDE1B1“ Cell Signaling 9(7): 519-529. 
2. Bender, A.T. et al., (2005). “Selective up-regulation of PDE1B2 upon monocyte-tomacrophage differentiation.” PNAS 102(2): 497-502.
3. Wunder, F. et al., (2009). “A novel PDE2A reporter cell line: characterization of the cellular activity of PDE inhibitors.” Mol. Pharm. 6(1): 326-36.