PDE1C TR-FRET Assay Kit

Catalog # 60705
Size: 96 reactions

DESCRIPTION: Phosphodiesterases (PDEs) play an important role in dynamic
regulation of cAMP and cGMP signaling. PDE1C is a calmodulin-dependent PDE that is
expressed principally in human myocardium. The PDE1C TR-FRET Assay Kit is
designed for identification of inhibitors of PDE1C using TR-FRET (Time Resolved
Fluorescence Resonance Energy Transfer) technology. The assay is based on the
generation of FAM-labeled nucleotide monophosphates by the PDE1C. These
phosphate groups bind to terbium-labeled nanoparticles, resulting in energy transfer
from the terbium to the FAM, which emits a fluorescent signal at 520 nm. The change in
fluorescent intensity can be easily measured using a fluorescence plate reader.

The PDE1C TR-FRET Assay Kit comes in a convenient 96-well format, with purified
PDE1C enzyme, fluorescently labeled PDE1C substrate (cAMP), binding agent, and
PDE assay buffer for 100 enzyme reactions. Using this kit, only two simple steps on a
microtiter plate are required for the PDE1C activity assay. First, the fluorescent-labeled
cAMP is incubated with a sample containing PDE1C for 1 hour. Second, a binding agent
and a terbium donor are added to the reaction mix and incubated for 30 minutes. Then,
fluorescence intensity can be measured using a fluorescence reader.

COMPONENTS:

APPLICATIONS: Great for screening small molecular inhibitors for drug discovery and
HTS applications.

STABILITY: At least 6 months from date of receipt when stored as directed.

REFERENCE(S): Vandeput. F., et al. J. Biol. Chem. 2007; 282(45): 32749-32757.

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