PDE3A TR-FRET Assay Kit

Catalog # 60706
Size: 96 reactions

DESCRIPTION: Phosphodiesterases (PDEs) play an important role in dynamic
regulation of cAMP and cGMP signaling. PDE3A, also known as cGMP-inhibited
phosphodiesterase, has been implicated in cardiovascular function and fertility.
The PDE3A TR-FRET Assay Kit is designed for identification of inhibitors of PDE3A
using TR-FRET (Time Resolved Fluorescence Resonance Energy Transfer) technology.
The assay is based on the generation of FAM-labeled nucleotide monophosphates by
the phosphodiesterase. These phosphate groups bind to terbium-labeled nanoparticles,
resulting in energy transfer from the terbium to the FAM, which emits a fluorescent signal
at 520 nm. The change in fluorescent intensity can be easily measured using a
fluorescence plate reader.

The PDE3A TR-FRET Assay Kit comes in a convenient 96-well format, with purified
PDE3A enzyme, fluorescently labeled PDE substrate (cAMP), binding agent, and PDE
assay buffer for 100 enzyme reactions. Using this kit, only two simple steps on a
microtiter plate are required for the PDE3A activity assay. First, the fluorescent-labeled
cAMP is incubated with a sample containing PDE3A for 1 hour. Second, a binding agent
and a terbium donor are added to the reaction mix and incubated for 1 hour. Then,
fluorescence intensity can be measured using a fluorescence reader.

APPLICATIONS: Great for screening small molecular inhibitors for drug discovery and
HTS applications.

STABILITY: 6 months from date of receipt when stored as directed.

COMPONENTS:

REFERENCE(S): Maurice, DH. Front. Biosci. 2005; 10:1221-8.

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