PD-L2 Inhibitor Screening Assay Kit
Catalog # 72006
Size: 96 reactions

DESCRIPTION: Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand
attenuates immune responses and is exploited by both tumors and viruses. The PD-L2
Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this
signaling. This kit comes in a convenient 96-well format, with biotin-labeled PD-1,
purified PD-L2, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
The key to this kit is the high sensitivity of detection of biotin-labeled PD-1 by
streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the
assay. First, PD-L2 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L2 on
the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an
HRP substrate to produce chemiluminescence, which can then be measured using a
chemiluminescence reader.

COMPONENTS:

APPLICATIONS: This kit is most useful for screening for inhibitors of PD-L2.

STABILITY: One year from date of receipt when stored as directed.

REFERENCES:
1. Lazar-Molnar, E., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 10483-10488.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.

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