Fluorogenic Prolyl OligoPeptidase (POP) Assay Kit
Catalog # 80106

Size: 96 reactions

DESCRIPTION: Prolyl oligopeptidase (POP), also known as prolyl endopeptidase
(PREP), is a cytosolic serine peptidase involved in the maturation and degradation of
peptide hormones and neuropeptides. The Fluorogenic Prolyl OligoPeptidase (POP)
Assay Kit is a complete assay system designed to measure activity of the purified POP
enzyme. It comes in a convenient 96-well format, with all the reagents necessary for 100
reactions. The Fluorogenic POP Activity Kit eliminates the dealing with radioactive
materials and chromatography in traditional assays. Purified human recombinant POP is
included in the kit as a positive control. Using this kit, only one simple step, in which the
fluorometric substrate is incubated with purified POP, is needed to analyze the POP
activity level. The resulting fluorescent product can then be easily measured with a
microtiter-plate fluorimeter.

COMPONENTS:

Note: The AMC standard is included so the researcher can quatitatively determine the
specific activity of the enzyme using the AMC standard as a measure of how much AMC
substrate was cleaved to release free AMC.

APPLICATIONS: Great for studying enzyme kinetics and screening small molecular
inhibitors for drug discovery and HTS applications.

REFERENCES:
1. Myohanen T.T. et al. (2007).Neurochem. Res. 32 (8): 1365-1374.
2. Garcia-Horsman J.A. et al. (2007). Scand. J. Gastroenterol. 42 (5): 562-571.

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