Renin Assay Kit
Catalog # 80211

Size: 96 reactions

DESCRIPTION: Renin is a protease that is synthesized by the kidneys. Renin catalyzes
the hydrolysis of angiotensinogen to angiotensin I, which is converted to angiotensin II, a
highly potent vasoconstrictor. Renin inhibitors have been used therapeutically in the
treatment of hypertension (high blood pressure). The Renin Assay Kit is designed to
measure Renin activity for screening and profiling applications. It comes in a convenient
96-well format, with purified, activated renin, renin substrate, and assay buffer for 100
enzyme reactions. Aliskiren Hydrochloride is also included as a control for renin
inhibition. The key to the Renin Assay Kit is the specific, fluorogenic substrate. Using this
kit, only one simple step on a microtiter plate is required for renin reactions. The
fluorometric substrate is incubated with a sample containing renin enzyme to produce a
fluorophore that can then be measured using a fluorescence reader. 

COMPONENTS:

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader

APPLICATIONS: Great for studying enzyme kinetics, screening small molecular
inhibitors for drug discovery and HTS applications.

STABILITY: 6 months from date of receipt when stored as directed.

REFERENCE(S):
1. Paschalidou, K. et al., Biochem. J. 2004; 382: 1031.
2. Gossas, T., et al., Naunyn Schmiedebergs Arch Pharmacol. 2012; 385(2):219-24.

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