MMP-1人基質(zhì)金屬蛋白酶1檢測(cè)試劑盒雙抗體夾心法檢測(cè)抗原，間接法檢測(cè)抗體，為客戶(hù)提供各種ELISA技術(shù)進(jìn)行樣本檢測(cè)。

英文縮寫(xiě)：MMP-1 Elisa kit

產(chǎn)品關(guān)鍵詞：基質(zhì)金屬蛋白酶1(MMP-1)酶聯(lián)免疫試劑盒

規(guī)格96T/48T

保存條件：2-8℃低溫保存

運(yùn)輸：快遞運(yùn)輸（可根據(jù)客戶(hù)要求，選擇具體的運(yùn)輸方式，無(wú)特殊要求我公司一般為順豐）。

MMP-1人基質(zhì)金屬蛋白酶1檢測(cè)試劑盒樣品準(zhǔn)備:

1. Acidify the plasma/serum with an equal amount of buffer A. For example, if you are using 1ml of plasma/serum, add 1 ml of buffer A. Mix and centrifuge at 6,000 to 17,000 x g for 20 minutes at 4°C.

2. Equilibrate a SEP-COLUMN containing 200 mg of C18 (Catalog No.: RK-SEPCOL-1) by washing with buffer B (1 ml, once) followed by buffer A (3 ml, 3 times).NOTE: From steps 3-5, no pressure should be applied to the column.

3. Load the acidified plasma/serum solution onto the pre-treated (Step 2) C-18 SEP-COLUMN.

4. Slowly wash the column with buffer A (3 ml, twice) and discard the wash.

5. MMP-1人基質(zhì)金屬蛋白酶1檢測(cè)試劑盒Elute the peptide slowly with buffer B (3 ml, once) and collect eluant into a polystyrene tube.

6. Evaporate eluant to dryness in a centrifugal concentrator or by a suitable substitute method.

7(i). For EIA kits, Fluorescent EIA kits and Chemiluminescent EIA kits, keep the dried extract at -20°C and perform the assay as soon as possible. For normal subject extracted from 1 ml original plasma/serum, use 125 ul 1x assay buffer to reconstitute the dried extract. Aliquot 50 ul into two designated assay wells (25 ul is left over). the concentration factor in this case is 8. (1 ml / 125 ul = 8). The original plasma peptide level is 1/8 of the level of final extracted plasma.

MMP-1人基質(zhì)金屬蛋白酶1檢測(cè)試劑盒樣本要求:

在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃，必須清楚要檢測(cè)的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測(cè)，對(duì)收集后當(dāng)天就進(jìn)行檢測(cè)的標(biāo)本，及時(shí)儲(chǔ)存在4℃?zhèn)溆茫�如有特殊原因需要周期收集�?biāo)本，請(qǐng)?jiān)炷Ｈ〔暮螅瑢��?biāo)本及時(shí)分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實(shí)驗(yàn)質(zhì)量，所以避免反復(fù)凍融。代測(cè)放免標(biāo)本的客戶(hù)取材前須向我司銷(xiāo)售人員索要說(shuō)明書(shū)，具體操作注意事項(xiàng)請(qǐng)與我司技術(shù)人員溝通。

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