Advantages of AASTYs in protein purification
Description
AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-50 - are highly-alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-50 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-50 show higher thermodynamic stability.
Every membrane protein solubilization needs to undergo a screening process in before. The characteristic phospholipid environment surrounding the different membrane proteins in question peforms differently well with each polymer. To support you in this process we offer a handy Screening Kit for AASTYs to test them all.
We recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022
Find the biotinylated version of this copolymer included in the NativeMP™ Biotin Kit.
Datasheets
Lab Results
As every membrane protein, surrounded by its characteristic phospholipid environment, performs differently well with every other polymer, it is generally advisable to screen for the most viable conditions of your protein of interest. However, when you're interested in conditions with lower polymer concentrations, AASTYs are a good choice to start with your experiments.
Fig. 1: AASTYs performance compared to other synthetic polymers. Especially at the lower concentration of 0.5% AASTY generally performs better than other polymers.
德國Cube Biotech中國獨家總代理：成都正民德思生物科技有限公司
訂購電話：400-9910-165 / 028-85568133
微信：zenmindes-sale
網址：www.zenmindes.com
AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-50 - are highly-alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-50 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-50 show higher thermodynamic stability.
Every membrane protein solubilization needs to undergo a screening process in before. The characteristic phospholipid environment surrounding the different membrane proteins in question peforms differently well with each polymer. To support you in this process we offer a handy Screening Kit for AASTYs to test them all.
We recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022
Find the biotinylated version of this copolymer included in the NativeMP™ Biotin Kit.
Datasheets
| FEATURE | |
| State | Lyophilized Powder, to be solved with water |
| Molecular Weight | 10-11 kDa |
| Buffer | HEPES |
| pH after solving | 7.5 |
| Divalent cationic tolerance | 6 mM |
| Acrylic Acid to Styrene Ratio | 50% to 50% |
| Shipping Temperature | ambient temperature |
| Storage of lyophilized copolymer | -20°C for several years |
| Storage of dissolved copolymer | 2-8°C for several days |
| Structure |  |
Lab Results
As every membrane protein, surrounded by its characteristic phospholipid environment, performs differently well with every other polymer, it is generally advisable to screen for the most viable conditions of your protein of interest. However, when you're interested in conditions with lower polymer concentrations, AASTYs are a good choice to start with your experiments.
Fig. 1: AASTYs performance compared to other synthetic polymers. Especially at the lower concentration of 0.5% AASTY generally performs better than other polymers.
德國Cube Biotech中國獨家總代理：成都正民德思生物科技有限公司
訂購電話：400-9910-165 / 028-85568133
微信：zenmindes-sale
網址：www.zenmindes.com
標簽：
蛋白純化;膜蛋白;AASTY
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